Protein & peptide sequencing

Peptide Mass fingerprint from MALDI-TOF MS and Peptide de novo sequencing from MS/MS spectrum

Proteins are excised and subjected to in-gel proteolytic digestion (e.g. trypsin) prior to sequence analysis by MS or MS/MS.

In the case of MALDI-TOF MS, peptide mass mapping techniques are used to create a unique mass fingerprint of the protein.

For protein identification by MS/MS, a single peptide ion is isolated and fragmented to form smaller product peptide ions that contain the amino acid information.

The protein is then identified based on these unique identifiers (mass mapping or tandem mass spectrum) by computer sequence database matching.

Sample requirements:
1,0 pmole/µl for proteolytic digestion
1,0 nmole for an isolated protein (sample must be pure)