PeptiPlak™

Antibody titration - Technical information - Price list

Peptiplaks are polystyrene microtiter plates with the antigen (peptide or protein) covalently bound to the wells of the plates. Peptiplaks are designed for immunoassays and enable results to be obtained in less than two hours. The amount of peptide coated in each well is 0,5 moles. The unique features of Peptiplaks make them an obvious choice for:

Determining antibody levels in the serum of immunized animal or in cell supernatant from hybridoma clones Affinity measurements by competition Elisa

Adhesion-molecule assays Enzyme-substrate studies Ligand-receptor interactions...

Antibody titration:

PeptiPlak immunoassays are:

• Performed within 2 hours
• Specific and sensitive
• Reproducible

PeptiPlaks:

• 96-well plate formats
• can be stored at 4°C for several months
• available with different terminal functionalities

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Technical Information

Peptides are covalently linked to PeptiPlaks according to an original method. Plates are available with 3 different functionalities for peptides by specific amino acids.

Methods of binding:

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Peptiplaks advantages:

Features		Functionalized polystyrene plate (96-well plates)
Binding		Covalent coupling
Use		Ready to use

Immunoassay:

Time		Results within 2 hours
Sensitivity		Superior to other plates in the market
Quantitative		Titration monitoring (fig.2)
Assay repeatability		Coefficient of variation < 7%

Others:

Multi titration		Detection of the binding of different antibodies to a single peptide, or of one antibody to different peptides
Robotic operations		Yes, possible

Technical Information

Figure 1: Comparison of antibody titration with PeptiPlak versus classical adsorption.		Figure 2: Measurement of serum antibody titers over time with PeptiPlak during an immunization protocol.

Figure 3: Multi titration, titration of 3 sera against 3 peptides bound to the same PeptiPlak.

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Prices

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